Hitrap Q

Hitrap Q. 4 to 30C 02 M Sodium Acetate in 20 Ethanol. HiTrap Q FF is prepacked with Q Sepharose Fast Flow and is a strong anion exchanger for small-scale protein purifications as well as screening of binding and elutions conditionsChromatography columns separate molecules within mixtures during gas or liquid chromatography. Prepacked with Q or SP Sepharose High Performance.

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Q Sepharose XL and SP Sepharose XL media have long chains of dextran coupled to a.

Application Separation of protein using a strong anion exchange medium prepacked in the HiTrap column format. HiTrap Capto Q ImpRes and Capto SP ImpRes columns provide fast reproducible and easy separations in a convenient format. Description HiTrap Q HP are prepacked ready-to-use Q Sepharose High Performance strong anion exchange columns for high-resolution small-scale protein purification.

HiTrap SP HP 1 ml and 5 ml HiTrap Q HP 1 ml and 5 ml HiTrap SP HP and HiTrap Q HP are prepacked ready to use cation and anion exchange columns for method scouting group separations sample concentration and sample clean-up of charged biomolecules. HiTrap affinity columns are ideal for method optimization or small scale purification of target proteins using well-established protocols. HiTrap SP HP and HiTrap Q HP are prepacked ready to use cation and anion exchange columns for method scouting group separations sample concentration and.

For applications where extremely low leakage during purification is critical. HiTrap Capto Q ImpRes and HiTrap Capto SP ImpRes are prepacked 1 ml and 5 ml columns for screening of selectivity binding and elution conditions as well as small scale purifications. Leakage of Ni2 from Ni Sepharose High Performance is very low under all normal conditions lower than for other IMAC media tested.

Soluble mite extract was separated using a 1 ml HiTRAP Q HP anion exchange column GE Healthcare and eluted with an increasing NaCl gradient. A PAGE separation of unbound column flow-through material and sequentially eluted fractions 125 obtained from the ion exchange chromatography of soluble mite extract from fed mites. Begin with a strong exchanger Q S SP to enable development work to be performed over a broad pH range.

HiTrap affinity columns HisTrap HP 1 ml and 5 ml HisTrap HP is a prepacked ready-to-use column for the preparative purification of His-tagged recombinant proteins by immobilized metal affinity chromatography IMAC using precharged Ni Sepharose High Performance. HiTrap SP XL and HiTrap Q XL provide fast reproducible and easy separations in a convenient format. HiTrap SP XL and HiTrap Q XL are 1 ml and 5 ml prepacked ready to use cation and anion exchange columns for method scouting group separations sample concentration and sample clean-up of charged biomolecules.

HiTrap Q FF is prepacked with Q Sepharose Fast Flow and is a strong anion exchanger for small-scale protein purifications as well as screening of binding and elutions conditions. HiTrap SP HP and HiTrap Q HP are prepacked ready to use cation and anion exchange columns for method scouting group separations sample concentration and sample clean-up of charged biomolecules. Use a strong anion exchanger Q to bind the proteins of interest if their isoelectric point is below pH 70 or unknown.

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